Izinto Zokwenza Izitsha Eziningi Ezithuthukisiwe (mIHC) Izinkitso

Isifiso sikaMultiplex Immunohistochemistry (mIHC) kuyinto ukuthi i-antigen kwiisekishi zithandwa kwi-antibody ethwesile. Emva kwaloo nto, i-antibody yesibini ethandwa nge-horseradish peroxidase (HRP) ithambisa ukuze ithande ne-primary antibody. Emva kokuthambisa i-tyramine fluorescence substrate, i-tyramine fluorescein substrates ezithile ezakhiwe zokwenza kwenziwa nguHRP-catalyzed hydrogen peroxide. Ekuboniseni i-fluorescein substrate, i-tyramine fluorescein substrate ezakhiwe ezakhiwe zokwenza kwenziwa nguHRP ekwakhiweni kwe-hydrogen peroxide. I-substrate ethandwa ikhwalitha ngokwe-covalently kwiiprotheni ezikwazi ukuthi zihlale zithandwa kwi-antigen-fluorescein.

Lezi dye zezifulethi zingathumela ngokwanele noma ngokubambisana, ezikwazi ukwenza ukuthi kube yimuphi umthetho we-fluorescence amplification kanye nokulabela kwesibindi esithandwa, ekwakheni okukhulu ukwakha okusezingeni eliphezulu.